Human Cardiomyocyte Differentiation Media 人心肌細胞分化培養基
產品型號： 017DK
i-HCm Differentiation Medium Kit is used to derive Cardiomyocytes (i-HCm) from Human Induced Pluripotent Stem Cells.
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From Skin Cells to Beating Heart Cells Dermal fibroblasts (HDF) isolated from human skin, reprogrammed to pluripotent stem cells (HiPSC), then differentiated to cardiomyocytes (i-HCm). (a) In vitro-cultured i-HCm immunofluorescently stained for Alpha Actinin (b) Troponin, and nuclei counter-stained for DAPI. (c) Pulsating heart tissue spheroid (organoid) constructed with Cyfuse Regenova Bio 3D Printer, incorporating (left) neonatal or (right) adult i-HCm & primary cardiac cells. Printed i-HCm derived from adult iPSC exhibit strong beating at 72 pulses per minute while neonatal i-HCm demonstrate weaker, faster beat rate. (d): Monolayer-cultured i-HCm beat spontaneously in rhythm.
DETAILS
HiPSC to i-HCm timeline using Differentiation Medium Kit (017DK)
i-HCm Differentiation Medium Kit, Xeno-Free (017DK)
| Cat. No. | Medium | Abbrev | Vol |
| 017D1-30 | Differentiation 1 | i-HCm DM1 | 30ml |
| 017D2-30 | Differentiation 2 | i-HCm DM2 | 30ml |
| 017D3-100 | Differentiation 3 | i-HCm DM3 | 100ml |
| 019-50 | Selection | i-HCm SM | 50ml |
| 021-30 | Maintenance | i-HCm MM | 30ml |
Store all kit components at 4°C. Stable for 4 weeks at 4ºC.
PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Human Cardiomyocyte Differentiation Medium: Differentiation, Selection & Maintenance Media sold together, packaged separately. See Details Tab. | 1 Kit | 017DK |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Human Cardiomyocyte Maintenance Medium: Maintenance medium for Human iPSC-Derived Cariomyocytes | 50 ml | 021-50 |
| Human Cardiomyoycte Plating Medium: Use for initial plating of Human iPSC-Derived Cardiomyocytes | 20 ml | 021P-20 |
EXTENDED FAMILY PRODUCTS
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HiPSC, from Adult Female HDF: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in Details Tab) | 1 Cryovial | iPS12-10 |
| Cryopreserved HiPSC, from Neonatal Male HDF: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in details) | 1 Cryovial | iPS11-10 |
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