Human Skeletal Muscle Cells: HSkMC 人體骨骼肌細胞:HSkMC

產品型號: 150K-05f

Human Skeletal Muscle Cells (HSkMC), isolated from adult or fetal donor limbs are positive for sarcomere myosin and can undergo differentiation to exhibit actin and myosin myofilaments.  Our HSkMC provide a useful model system to study many aspects of muscular function and disease.  Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, so select lots undergo an additional set of Pre-Screening at Cell Applications, Inc. to demonstrate AMPK signaling and responsiveness to insulin stimulation.
 
HSkMC from Cell Applications, Inc. have been used to:
  • Serve as a differentiated control in a study of developmental regulator genes in hESC
  • Characterize statin-induced gene expression changes and demonstrate cytotoxic effect of statins in skeletal muscle cells
  • Identify molecular mechanisms of mitochondrial myopathy and sideroblastic anemia resulting from a missense mutation in the PUS1 gene
  • Investigate chemokine-like factor expression in the idiopathic inflammatory myopathies
  • Demonstrate that fasting activates AceCS2 gene expression by inducing KLF15 transcription factor
  • Characterize human FGFR3-positive sarcoma-initiating stem cells
  • Identify trans-Golgi network proteins and Notch and Hedgehog pathways as putative targets for rhabdomyosarcoma therapy
  • Show that riluzole muscle relaxant effects are mediated by inhibition of INa and stimulation of BKC-channel activity
  • Develop nitric esters that combine the pharmacological functions of NO and muscle relaxation properties for treatment of muscular diseases
  • Develop biodegradable polymer-based transgene delivery vectors for muscular dystrophy treatment
  • Design optimal coating for orthopedic metallic implants

DETAILS

Tissue
Normal healthy human limb skeletal muscle
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Multinucleated myotubes form after culture in fusion medium
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HSkMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HSkMC (150-05), Growth Medium (151-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Tsfr Med, psg 3, flasks or plates
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

PRODUCTS

 

PRODUCT SIZE CAT.#
Cryopreserved HSkMC, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 150K-05f
Cryopreserved HSkMC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 150K-05a
Cryopreserved HSkMC, Pre-Screened, Fetal: 5x10^5 Cells (Pre-Screened, Fetal), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit S150K-05f
Cryopreserved HSkMC, Adult: Frozen HSkMC (5x10^5) 1 Cryovial 150-05a
Cryopreserved HSkMC, Fetal: Frozen HSkMC (5x10^5) 1 Cryovial 150-05f
Cryopreserved HSkMC, Pre-Screened, Fetal: Frozen HSkMC (5x10^5) 1 Cryovial S150-05f
Cryopreserved HSkMC-COPD, Pre-Scr, Adult: 5x10^5 Cells (from donor with COPD, Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit S150COPDK-05a
Cryopreserved HSkMC-COPD, Pre-Scr, Adult: Frozen HSkMC-COPD from donor with Chronic Obstructive Pulmonary Disease, Adult (5x10^5) 1 Cryovial S150COPD-05a
Cryopreserved HSkMC-T2D, Adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 150T2DK-05a
Cryopreserved HSkMC-T2D, Adult: Frozen HSkMC from donor with Type 2 Diabetes (5x10^5) 1 Cryovial 150T2D-05a
Proliferating HSkMC, Adult: Actively growing, dividing cells in medium T-25 Flask 151-25a
Proliferating HSkMC, Adult: Actively growing, dividing cells in medium T-75 Flask 151-75a
Proliferating HSkMC, Fetal: Actively growing, dividing cells in medium T-25 Flask 151-25f
Proliferating HSkMC, Fetal: Actively growing, dividing cells in medium T-75 Flask 151-75f
Proliferating HSkMC, Pre-Screened, Fetal: Actively growing, dividing cells in medium T-25 Flask S151-25f
Proliferating HSkMC, Pre-Screened, Fetal: Actively growing, dividing cells in medium T-75 Flask S151-75f

 

RELATED PRODUCTS

 

PRODUCT SIZE CAT.#
HSkMC Growth Medium: All-in-one ready-to-use 500 ml 151-500
HSkMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Yields 500 ml 151K-500
HSkMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. 500 ml 150-500
HSkMC Growth Supplement: Added to Basal Medium to create Growth Medium 50 ml (2 parts) 151-GS
HSkMC Differentiation Medium: Promotes cells to change from one type to another, more specialized 250 ml 151D-250

 

EXTENDED FAMILY PRODUCTS

 

PRODUCT SIZE CAT.#
Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody 100 ul CP10325
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. 50 ml 040-50
Cyto-X Cell Counting Reagent: 500 tests 1 Bottle 028-01
Cyto-X Cell Counting Reagent Sample: 100 tests Sample 028-S
HSkMC RNA, Adult: Total RNA prepared from Human Skeletal Muscle Cells, adult 10 ug 150-R10a
HSkMC RNA, Adult: Total RNA prepared from Human Skeletal Muscle Cells, adult 25 ug 150-R25a
HSkMC RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, fetal 10 ug 150-R10f
HSkMC RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, fetal 25 ug 150-R25f
HSkMC, Multinucleated Myotubes RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, Differentiated, fetal 10 ug 150D-R10f
HSkMC, Multinucleated Myotubes RNA, Fetal: Total RNA prepared from Human Skeletal Muscle Cells, Differentiated, fetal 25 ug 150D-R25f
Human Interleukin-6 (IL-6): Human Interleukin-6 20 ug RP1010-20
Human Interleukin-6 (IL-6): Human Interleukin-6 100 ug RP1010-100
Human Interleukin-6 (IL-6): Human Interleukin-6 1000 ug RP1010-1000
Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue 50 ug 1H60-50
Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue 250 ug 1H60-250
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 1 Kit 090K
Mouse Myostatin Antibody: Mouse Myostatin Antibody 100 ul CP10315
Human Myostatin (GDF-8): Human Myostatin 10 ug RP1022-10
Human Myostatin (GDF-8): Human Myostatin 100 ug RP1022-100
Human Myostatin (GDF-8): Human Myostatin 1000 ug RP1022-1000
Human Myostatin Propeptide: Human Myostatin Propeptide 25 ug RP1142-25
Human Myostatin Propeptide: Human Myostatin Propeptide 100 ug RP1142-100
Human Myostatin Propeptide: Human Myostatin Propeptide 1000 ug RP1142-1000
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free 20 ug RP1010AF-20
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free 100 ug RP1010AF-100
Human IL-6, Animal Free: Human Interleukin-6, Animal-Free 1000 ug RP1010AF-1000
Skeletal Muscle Cell Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment 250 ml 151S-250
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