Human Osteoblasts: HOB 人成骨細胞: HOB
產品型號： 406K-05a
Human Osteoblasts (HOb) from Cell Applications, Inc. are Isolated from fetal or adult human bones. The cells, positive for bone mineralization, provide an excellent model system for studies relevant to the skeletal system. Additionally, osteoblasts from osteoarthritis or rheumatoid arthritis donors are available.
Our HOb have been used to examine:
- Actions, signaling pathways and cross-talk of growth factors, cytokines and interleukins
- Nuclear factors and gene expression
- Cell proliferation and differentiation
- Bone formation, morphogenesis, and bone morphogenic proteins
- Mechanisms behind bone disorders and defects including bone loss, osteoporosis, osteoclast inhibition, periodontitis, arthritis and related matrix metalloproteinase production
- Cancers, such as osteosarcoma and oncogenes. For the pharmaceutical industry, the cells have been pivotal in developing
- New pharmaceutical treatment strategies and drug delivery methods
- Orthopedic implants and tissue engineering have also been positively impacted by osteoblast research
DETAILS
|
Tissue
|
Normal healthy human bone
|
| QC |
No bacteria, yeast, fungi, mycoplasma, virus
|
| Character |
Bone mineralization (Kossa stain)
|
| Bioassay |
Attach, spread, proliferate in Growth Med
|
| Cryovial |
500,000 HOb (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
|
| Kit |
Cryovial frozen HOb (406-05), Growth Medium (417-500), Subcltr Rgnt Kit (090K)
|
| Proliferating |
Shipped in Gr Med, 3rd psg (flasks or plates)
|
| Doublings |
At least 10
|
| Applications |
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|
PRODUCT
| PRODUCT | SIZE | CAT.# |
| Cryopreserved HOb, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | 1 Kit | 406K-05a |
| Cryopreserved HOb, Adult: Frozen HOb (5x10^5) | 1 Cryovial | 406-05a |
| Cryopreserved HOb, Fetal: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for Specifics) | 1 Kit | 406K-05f |
| Cryopreserved HOb, Fetal: Frozen HOb (5x10^5) | 1 Cryovial | 406-05f |
| Proliferating HOb, Adult: Actively growing, dividing cells, in medium | T-25 Flask | 407-25a |
| Proliferating HOb, Adult: Actively growing, dividing cells, in medium | T-75 Flask | 407-75a |
| Proliferating HOb, Fetal: Actively growing, dividing cells, in medium | T-25 Flask | 407-25f |
| Proliferating HOb, Fetal: Actively growing, dividing cells, in medium | T-75 Flask | 407-75f |
RELATED PRODUCTS
| PRODUCT | SIZE | CAT.# |
| HOb Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | 500 ml | 416-500 |
| HOb Differentiation Medium: Promotes cells to change from one type to another, more specialized | 250 ml | 417D-250 |
| HOb Growth Medium: All-in-one ready-to-use | 500 ml | 417-500 |
| HOb Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Yields 500 ml | 417K-500 |
| HOb Growth Medium, wo FBS: Growth medium without FBS | 500 ml | 417F-500 |
| HOb Growth Supplement: Added to Basal Medium to create Growth Medium | 50 ml | 417-GS |
| EXTENDED FAMILY PRODUCTS | ||
| PRODUCT | SIZE | CAT.# |
| Mouse BMPR2 Antibody: Mouse BMPR2 Antibody | 100 ul | CP10368 |
| Monoclonal Bone Morphogenic Protein 4, BMP-4, Antibody: Monoclonal Bone Morphogenic Protein 4, BMP-4, Antibody | 100 ul | CB12470 |
| Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 | 10 ug | RP1053-10 |
| Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 | 100 ug | RP1053-100 |
| Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 | 1000 ug | RP1053-1000 |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | 20 ug | RP1060-20 |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor | 100 ug | RP1060-100 |
| Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Facto | 1000 ug | RP1060-1000 |
| Cyto-X Cell Counting Reagent: 500 tests | 1 Bottle | 028-01 |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Sample | 028-S |
| HOb RNA, Adult: Total RNA prepared from Human Osteoblasts, adult | 10 ug | 406-R10a |
| HOb RNA, Adult: Total RNA prepared from Human Osteoblasts, adult | 25 ug | 406-R25a |
| HOb RNA, Fetal: Total RNA prepared from Human Osteoblasts, fetal | 10 ug | 406-R10f |
| HOb RNA, Fetal: Total RNA prepared from Human Osteoblasts, fetal | 25 ug | 406-R25f |
| HOb Differentiated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Differentiated, fetal | 10 ug | 406D-R10f |
| HOb Differentiated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Differentiated, fetal | 25 ug | 406D-R25f |
| HOb Stimulated RNA, Adult: Total RNA prepared from Human Osteoblasts, Stimulated, adult | 10 ug | 406S-R10a |
| HOb Stimulated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Stimulated, fetal | 10 ug | 406S-R10f |
| HOb Stimulated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Stimulated, fetal | 25 ug | 406S-R25f |
| HOb Stimulated RNA, Adult: Total RNA prepared from Human Osteoblasts, Stimulated, adult | 25 ug | 406S-R25a |
| Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand | 10 ug | RP1019-10 |
| Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand | 100 ug | RP1019-100 |
| Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand | 1000 ug | RP1019-1000 |
| Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free | 10 ug | RP1019AF-10 |
| Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free | 100 ug | RP1019AF-100 |
| Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free | 1000 ug | RP1019AF-1000 |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | 1 KIT | 090K |
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