Human Bronchial Epithelial Cells: HBEpC 人支氣管上皮細胞

產品型號: 502K-05A_502-05A

HBEpC from Cell Applications, Inc. have been used to examine:
  • Activation, expression and production of genes, kinases and signaling pathways by cytokines, growth factors, interleukins, binding proteins and pro-inflammatory molecules. 
  • Stimulation-dependent, observable changes in proliferation, bronchial epithelial permeability, crosslinking of membrane glycoproteins and cell surface adhesion molecules.  Drug discovery cell screening for in vitro assay of compounds, or to extend and confirm high-throughput work done in cell lines.
  • Clinical focused discoveries leveraging HBEpC include therapeutics to suppress tumor gene transcription, apoptosis, inflammation, auto-immune disease and viral infection, while enhancing cell protection, repair and lifespan.

DETAILS

Tissue Human bronchial epithelium: nondiseased, Asthma, COPD, or Type 2 Diabetes
QC No bacteria, yeast, fungi, mycoplasma, virus
Bioassay Attach, spread, proliferate in Growth Med
Cryovial 500,000 HBEpC (1st passage) frozen in Basal Med w/ 10% FBS, 10% DMSO
Kit Cryovial frozen HBEpC (502-05a), Gr Med (511-500), Subculture Rgnt Kit (090K)
Proliferating Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings At least 16
Applications Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

PRODUCTS

PRODUCT SIZE CAT.#
Cryopreserved HBEpC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 502K-05a
Cryopreserved HBEpC, Adult: Frozen HBEpC (5x10^5) 1 Cryovial 502-05a
Cryopreserved HBEpC-AS, Adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 502ASK-05a
Cryopreserved HBEpC-AS, Adult: Frozen HBEpC-AS from donor with Asthma (5x10^5) 1 Cryovial 502AS-05a
Cryopreserved HBEpC-COPD, Adult: 5x10^5 Cells (from donor with COPD, Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 502COPDK-05a
Cryopreserved HBEpC-COPD, Adult: Frozen HBEpC-COPD from donor with Chronic Obstructive Pulmonary Disease (5x10^5) 1 Cryovial 502COPD-05a
Cryopreserved HBEpC-T2D, Adult: 5x10^5 Cells (from donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 502T2DK-05a
Proliferating HBEpC, Adult: Actively growing, dividing cells in medium T-25 Flask 503-25a
Proliferating HBEpC, Adult: Actively growing, dividing cells in medium T-75 Flask

503-75a

RELATED PRODUCTS    
PRODUCT SIZE CAT.#
HBEpC/HTEpC Growth Medium: All-in-one ready-to-use. Cat# 511-500 fully supports cell growth on its own. Retinoic acid (RA) is a optional component; one vial of RA goes into 500ml media. Some researchers may elect to include RA if studies include cell differentiation or pseudo-stratification. 500 ml 511-500
HBEpC/HTEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Kit 511K-500
HBEpC/HTEpC Basal Medium: Without growth supplement (GS).  Add GS before use. 500 ml 510-500
HBEpC/HTEpC Growth Supplement: Added to Basal Medium to create Growth Medium 5 ml 511-GS
HBEpC/HTEpC Differentiation Medium: Promotes cells to change from one type to another, more specialized 250 ml 511D-250
HBEpC/HTEpC Growth Medium wo Antibiotics: Growth medium without antibiotics 500 ml 511A-500

 

EXTENDED FAMILY PRODUCTS    
PRODUCT SIZE CAT.#
3-D Airway Model: HBEpC differentiated into pseudostriated epithelium on PCF inserts. Available only in the US. 12 Inserts 502-3D-12
3-D Airway Model: HBEpC differentiated into pseudostriated epithelium on PCF inserts. Available only in the US. 24 Inserts 502-3D-24
3-D Airway Model : Cells, Media, Reagents & Inserts (See Details tab for specifics) 1 Kit 502K-3D
Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody 100 ul CB19846
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO 50 ml 040-50
Cyto-X Cell Counting Reagent: 500 tests 1 Bottle 028-01
Cyto-X Cell Counting Reagent Sample: 100 tests Sample 028-S
Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns 1 Kit TF102K
Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns 1 Sample Kit TF102KS
Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 100 ug RP1026-100
Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 500 ug RP1026-500
Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor 1000 ug RP1026-1000
Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 100 ug RP1026AF-100
Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 500 ug RP1026AF-500
Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free 1000 ug RP1026AF-1000
HBEpC RNA, Adult: Total RNA prepared from Human Bronchial Epithelial Cells, adultt 10 ug 502-R10a
HBEpC RNA, Adult: Total RNA prepared from Human Bronchial Epithelial Cells, adult 25 ug 502-R25a
Human Lung RNA: Total RNA prepared from human lung tissue 50 ug 1H40-50
Human Lung RNA: Total RNA prepared from human lung tissue 250 ug 1H40-250
Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha 10 ug RP1173-10
Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha 100 ug RP1173-100
Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha 1000 ug RP1173-1000
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 1 Kit 090K
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