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    Human Peripheral Blood Monocytes: HPBM 人外周血單核細胞：HPBM

    產品型號： 6906K-50a

    Human Peripheral Blood Monocytes (HPBM) provide a useful in vitro tool for studying various aspects of pathology and biology.  Our CD14+ Monocytes are isolated and purified from human peripheral blood of healthy donors and cryopreserved immediately after isolation.
    HPBM are the precursors of tissue macrophages and dentritic cells and are central to immunological responses, including cell-mediated cytotoxicity and production of inflammatory mediators.  The CD14 monocyte subset expresses a component of the lipopolysaccharide (LPS) receptor complex, are large with diameter of  ~18 μm, represent ~80%–90% of circulating monocytes, and express higher levels of CD62L (L-selectin) and CD11b (also referred to as Mac-1 or CR3) and lower levels of MHC class II than do CD16+ monocytes.
    HPBM from Cell Applications, Inc. have been used to
    • Investigate anti-oxidant activity of NF-E2-related transcription factor 2 (NRF2)
    • Identify aldo-keto reductase (AKR) as a tightly regulated human target of NRF2 that can be used as a marker of NRF2 activity
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    DETAILS

    Tissue
    Normal healthy human peripheral blood
    QC
    		 No bacteria, yeast, fungi, mycoplasma, virus
    Character
    		 CD14 positive selection
    Cryovial
    5,000,000 HPBM frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO
    Kit
    Cryovial Frozen HPBM (6906-50a), Maintenance Medium (615-250)
    Doublings N/A - Blood cells don't proliferate in vitro
    Applications Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

     

    PRODUCTS

     

    PRODUCT SIZE CAT.#
    Cryopreserved HPBM, Adult: 5x10^6 Cells CD14+ Cells (Adult) & Medium (See Details tab for specifics) 1 Kit 6906K-50a
    Cryopreserved HPBM, Adult: Frozen HPBM, CD14+ 1 Cryovial 6906-50a

     

    RELATED PRODUCTS

     

    PRODUCT SIZE CAT.#
    Human Blood Cell Culture Medium: Optimized and ready for use. 250 ml 615-250
    Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately Yields 250 ml 615K-250
    Human Blood Cell Basal Medium: Basal medium (contains no growth supplement).  Add Supplement before use. 250 ml 614-250
    Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium 40 ml 615-CS
    Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells 50 ml 040-50BC

     

    EXTENDED FAMILY PRODUCTS

     

    PRODUCT SIZE CAT.#
    Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells 50 ml 040-50BC
    Cyto-X Cell Counting Reagent: 500 tests 1 Bottle 028-01
    Cyto-X Cell Counting Reagent Sample: 100 tests Sample 028-S
    Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand 10 ug RP1021-10
    Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand 100 ug RP1021-100
    Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand 1000 ug RP1021-1000
    Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor 20 ug RP1008-20
    Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor 100 ug RP1008-100
    Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor 1000 ug RP1008-1000
    Human Interleukin-1-Beta (IL-1 beta): Human Interleukin-1-beta 10 ug RP1167-10
    Human Interleukin-1-Beta (IL-1 beta): Human Interleukin-1-beta 100 ug RP1167-100
    Human Interleukin-1-Beta (IL-1 beta): Human Interleukin-1-beta 1000 ug RP1167-1000
    Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 25 ug RP1127-25
    Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 100 ug RP1127-100
    Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 1000 ug RP1127-1000
    Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Factor-alpha 10 ug RP1111-10
    Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Factor-alpha 1000 ug RP1111-1000
    Human Macrophage Colony Stimulating Factor (M-CSF): Human Macrophage-Colony Stimulating Factor 10 ug RP1003-10
    Human Macrophage Colony Stimulating Factor (M-CSF): Human Macrophage-Colony Stimulating Factor 100 ug RP1003-100
    Human Macrophage Colony Stimulating Factor (M-CSF): Human Macrophage-Colony Stimulating Factor 1000 ug RP1003-1000
    Human Monocyte Chemotactic Protein-1 (MCP-1/CCL2): Human Monocyte Chemotactic Protein-1 20 ug RP1013-20
    Human Monocyte Chemotactic Protein-1 (MCP-1/CCL2): Human Monocyte Chemotactic Protein-1 100 ug RP1013-100
    Human Monocyte Chemotactic Protein-1 (MCP-1/CCL2): Human Monocyte Chemotactic Protein-1 1000 ug RP1013-1000
    Human Monocyte Chemotactic Protein-2 (MCP-2 / CCL8): Human Monocyte Chemotactic Protein-2 10 ug RP1130-10
    Human Monocyte Chemotactic Protein-2 (MCP-2 / CCL8): Human Monocyte Chemotactic Protein-2 100 ug RP1130-100
    Human Monocyte Chemotactic Protein-2 (MCP-2 / CCL8): Human Monocyte Chemotactic Protein-2 1000 ug RP1130-1000
    Human Monocyte Chemotactic Protein-3 (MCP-3 / CCL7): Human Monocyte Chemotactic Protein-3 10 ug RP1131-10
    Human Monocyte Chemotactic Protein-3 (MCP-3 / CCL7): Human Monocyte Chemotactic Protein-3 100 ug RP1131-100
    Human Monocyte Chemotactic Protein-3 (MCP-3 / CCL7): Human Monocyte Chemotactic Protein-3 1000 ug RP1131-1000
    Human Monocyte Chemotactic Protein-4 (MCP-4 / CCL13): Human Monocyte Chemotactic Protein-4 20 ug RP1132-20
    Human Monocyte Chemotactic Protein-4 (MCP-4 / CCL13): Human Monocyte Chemotactic Protein-4 100 ug RP1132-100
    Human Monocyte Chemotactic Protein-4 (MCP-4 / CCL13): Human Monocyte Chemotactic Protein-4 1000 ug RP1132-1000
    Human M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free 1000 ug RP1003AF-1000
    Human M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free 100 ug RP1003AF-100
    Human M-CSF, Animal-Free: Human Macrophage-Colony Stimulating Factor, Animal-Free 10 ug RP1003AF-10
    Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free 20 ug RP1008AF-20
    Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free 100 ug RP1008AF-100
    Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free 1000 ug RP1008AF-1000
    Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free 10 ug RP1021AF-10
    Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free 100 ug RP1021AF-100
    Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free 1000 ug RP1021AF-1000
    Human Tumor Necrosis Factor Alpha (TNF-alpha): Human Tumor Necrosis Facto-alpha 100 ug RP1111-100
    Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free 10 ug RP1111AF-10
    Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free 100 ug RP1111AF-100
    Human TNF-alpha, Animal-Free: Human Tumor Necrosis Factor-alpha, Animal-Free 1000 ug RP1111AF-1000
    Human IL-1b, Animal-Free: Human Interleukin-1β, Animal-Free 10 ug RP1167AF-10
    Human IL-1b, Animal-Free: Human Interleukin-1β, Animal-Free 100 ug RP1167AF-100
    Human IL-1b, Animal-Free: Human Interleukin-1β, Animal-Free 1000 ug RP1167AF-1000

    General Instructions for Culturing Human Periphery Blood CD14+ Monocyte Cells (HPBM)

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