Human Hepatic Stellate Cells: HHSC人肝星狀細胞:HHSC

產品型號: 782K-05f

Hepatic Stellate Cells (HHSC) are one of the major cell type that reside between hepatocytes and blood vessels in the liver. HHSCs play an important role in liver homeostasis, maintenance and regeneration including retinol metabolism. Activation of stellate cells in disease state leads to liver fibrosis, hepatitis B & C, fatty liver disease, diabetes and other chronic diseases. Hepatic Stellate Cells are primary cells derived from human liver and cultured in optimized Hepatic Stellate Cells Growth Medium. Primary culture of Hepatic Stellate Cells is suitable for many cell-based assays including toxicity, drug screening and metabolism and is a valuable tool to study liver function, physiology and liver diseases
 

Characterization: Positive for α-smooth muscle actin and fibronectin by ICC staining.

DETAILS

Tissue Normal healthy human liver tissue
QC No bacteria, yeast, fungi, mycoplasma, virus
Bioassay Attach, spread, proliferate in Growth Med
Cryovial 500,000 HHSC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit Cryovial frozen HHSC (782-05f), Growth Medium (7117-500), Subcltr Rgnt Kit (090K)
Proliferating Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings At least 15
Applications Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 

PRODUCTS

 

PRODUCT SIZE CAT.#
Cryopreserved HHSC, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 782K-05f
Cryopreserved HHSC, fetal: Frozen HHSC (5x10^5) 1 Cryovial 782-05f
Proliferating HHSC, fetal: Actively growing, dividing cells in medium T-25 Flask 783-25f
Proliferating HHSC, fetal: Actively growing, dividing cells in medium T-75 Flask 783-75f

 

RELATED PRODUCTS

 

PRODUCT SIZE CAT.#
Human Hepatic Stellate Cells Growth Medium: All-in-one ready-to-use 500 ml 7117-500
Human Hepatic Stellate Cells Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Yields 500 ml 7117K-500
Human Hepatic Stellate Cells Growth Medium Supplement: Added to Basal Medium to create Growth Medium 50 ml 7117-GS
Human Hepatic Stellate Cells Basal Medium: Basal medium (contains no growth supplement). Add GS before use. 500 ml 7116-500

 

EXTENDED FAMILY PRODUCTS

 

PRODUCT SIZE CAT.#
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 1 Kit 090K
Cyto-X Cell Counting Reagent: 500 tests 1 Bottle 028-01
Cyto-X Cell Counting Reagent Sample: 100 tests Sample 028-S
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