Normal Human Umbilical Vein Endothelial Cells (HUVEC), Primary 人類原代臍靜脈內皮細胞

Lifeline® normal Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Lifeline® VascuLife® Medium, provide an ideal low serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology.

• Lifeline®HUVEC may be cultured with or without VEGF.
• HUVEC can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife® medium.

Lifeline® HUVEC are isolated from human umbilical cords, and cryopreserved as primary cells to ensure the highest viability and plating efficiency.

• HUVEC are isolated in VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
• Our HUVEC are quality tested in VascuLife® 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
• Lifeline® Umbilical Vein Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

500,000 cells per vial

Lifeline® umbilical vein endothelial cells have been quality tested for:

• Proliferation and Morphology:  Normal cell appearance for 15 population doublings
• Cell Viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility:  Negative for mycoplasma. Negative for bacterial and fungal growth
• Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR
• Specific Staining:  von Willebrand Factor positive. Smooth muscle a-actin negative.