Epidermal Melanocytes — Neonatal 人體表皮黑色素細胞—新生兒

Lifeline® normal Human Epidermal Melanocytes neonatal (HEMn), when grown in Lifeline DermaLife® M, provide an ideal low serum culture model, without PMA or Cholera toxin, for the accurate testing of compounds and for use in the study of cancer or dermal biology.

Lifeline® Melanocytes are cryopreserved as primary cells to ensure the highest viability and plating efficiency.

Our Human Epidermal Melanocytes are quality tested in DermaLife M Medium to ensure optimal reduced-serum growth and morphology over a period of at least 15 population doublings at rates greater than media supplemented with higher serum, PMA or Cholera toxin.

Lifeline® HEM are not exposed to antimicrobials or phenol red when cultured in Lifeline® DermaLife M melanocyte cell culture medium,  an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements, but they are not needed or recommended to achieve optimal cell performance in most research applications.

500,000 cells per vial

CELL FEATURES:

• HEM provide an ideal model for testing of compounds, and in the study of cancer or dermal biology.
• Culture HEM without PMA or Cholera toxin or other artificial non-specific stimulants. 
• HEM are extensively tested for quality and optimal performance.
• Lifeline guarantees performance and quality. 

NORMAL HUMAN EPIDERMAL MELANOCYTES ARE TESTED FOR:

• Cell Count: 500,000 cryopreserved cells per vial 
• Low Serum: Proliferation Population growth at various densities
• Morphology: Normal cell appearance for 15 population doublings (HEMn and HEMn-HP), or 10 population doublings (HEMa and HEMa-HP)
• Cell Viability: Minimum 70% viability when thawed from cryopreservation
• Sterility Testing: Negative for mycoplasma, bacterial or fungal growth for 14 days
• Virus Testing: Negative for HIV, HBV, and HCV by PCR
• Specific Testing: L- Dopa Oxidase Activity Assay