Human Coronary Artery Smooth Muscle Cells 人體冠狀動脈平滑肌細胞

產品型號: FC-0031

Lifeline® normal Human Coronary Artery Smooth Muscle Cells (HCASMC), when grown in VascuLife® SMC Medium, provide an ideal low serum (5%) culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.

Lifeline® Coronary Artery Smooth Muscle Cells are Primary Cells that have been isolated from human coronary arteries, plated and expanded in culture vessels twice before being harvested for cryopreservation to ensure the highest purity, viability and plating efficiency.

Coronary artery smooth muscle cells, 100X

Coronary artery smooth muscle cells, 100X

Our Human Smooth Muscle Cells are quality tested in VascuLife® SMC Medium and demonstrate optimal low serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented media.

Lifeline® coronary artery smooth muscle cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® SMC Medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements; however, they are not needed, or recommended, to achieve optimal cell performance in most research applications.

  • Coronary artery smooth muscle cells can be grown without phenol red or antimicrobials when cultured in VascuLife® SMC Medium.

500,000 cells per vial

NORMAL HUMAN ENDOTHELIAL CELLS ARE TESTED FOR:

                          

Cell Count  500,000 cryopreserved cells per vial
Proliferation and Morphology Normal cell appearance for 15 population doublings
Cell Viability Minimum 70% viability when thawed from cryopreservation
Sterility

Testing Negative for mycoplasma

Negative for bacterial and fungal growth

Virus Testing Negative for HIV-1, HIV-2, HBV, and HCV by PCR
Specific Staining

von Willebrand Factor negative

Smooth muscle α-actin positive after differentiation

 

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