Human Dermal Fibroblasts – Neonatal, Xeno-Free, Primary 人類皮膚纖維母細胞—新生兒、無異種、原代

Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human pluripotent stem cell cultures or as a model to study wound healing, toxicology or basic cell biology. They have not been exposed to any non-human or animal material.

Human Dermal Fibroblasts-Neonatal, passage 2, 5 days after inoculation with 2000 cells/cm2 (100X)

HDFn-XF are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HDFn-XF are quality tested in FibroLife® Xeno-Free Complete Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media containing non-human proteins or supplements.

Lifeline® HDFn-XF are not exposed to antimicrobials or phenol red when cultured in FibroLife® Xeno-Free Complete Medium, an advantage since these supplements can cause cell stress and ” masking effects” that may negatively impact experimental results.

500,000 cells per vial

Neonatal dermal fibroblasts are tested for:

• Proliferation and morphology:  Normal cell appearance for 15 population doublings
• Cell viability:  Minimum 70% viability when thawed from cryopreservation
• Sterility:  Negative for mycoplasma.  Negative for bacterial and fungal growth
• Virus:  Negative for HIV-1, HIV-2, HBV, and HCV by PCR

Lifeline® documents that all materials used in the manufacture of products which are labeled “Xeno-Free” have never come into contact with material of animal (non-human) origin and are not of animal (non-human) origin.

CELL FEATURES:

• HDFn-XF are cryopreserved as primary cells, e.g. cells are isolated from human foreskin and expanded in culture vessels once before being harvested for cryopreservation.
• HDFn-XF are not exposed to phenol red or antimicrobials when cultured in FibroLife Xeno-Free Complete Medium.
• HDFn-XF are not exposed to any animal§-derived material during isolation or when cultured in FibroLife Xeno-Free Complete Medium.
• HDFn-XF are extensively tested for quality and optimal performance and Xeno-free status of all materials used in the isolation, expansion and cryopreservation of each lot is documented.
• Lifeline guarantees performance and quality.